In Silico prediction of mutant HIV-1 proteases cleaving a target sequence

In Silico Prediction of Mutant HIV-1 Proteases Cleaving a

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We found two mutant proteases as best candidates for specificity and cleavability towards the target sequence. Introduction Proteases represent a class of enzymes ubiquitous in all living organisms, with multiple applications in industry and biotechnology research [1] – [3] .

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In SilicoPrediction of Mutant HIV-1 Proteases Cleaving a

We found two mutant proteases as best candidates for specificity and cleavability towards the target sequence. Citation: Jensen JH, Willemoe¨s M, Winther JR, De Vico L (2014) In Silico Prediction of Mutant HIV-1 Proteases Cleaving a Target Sequence.

In Silico Prediction of Mutant HIV-1 Proteases Cleaving a

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¨ s M, Winther JR, De Vico L (2014) In Silico Prediction of Mutant HIV-1 Proteases Cleaving a Target Sequence. PLoS ONE 9(5): e95833.

In Silico Prediction of Mutant HIV-1 Proteases Cleaving a

Secondly, we show an algorithm we developed to predict mutant HIV-1 proteases capable of cleaving a new target substrate peptide, different from the natural targets of HIV-1 protease. The obtained in silico mutant enzymes were analyzed in terms of cleavability and specificity towards the target peptide using the energy-only methodology.

In silico prediction of mutant HIV-1 proteases cleaving a

Secondly, we show an algorithm we developed to predict mutant HIV-1 proteases capable of cleaving a new target substrate peptide, different from the natural targets of HIV-1 protease. The obtained in silico mutant enzymes were analyzed in terms of cleavability and specificity towards the target peptide using the energyonly methodology.

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prediction of mutant HIV-1 proteases cleaving a target

In silico prediction of mutant HIV-1 proteases cleaving a target sequence WT-PR Wild type HIV-1 protease. mutant PR Mutant HIV-1 protease. Pr3 set Setofmutant proteases derived fromPr3, a mutant protease developed by Alvizo etal. These were heterodimer proteases.

Structure at 2.5-.ANG. resolution of chemically

In Silico Prediction of Mutant HIV-1 Proteases Cleaving a Target Sequence. Jan H. Jensen, Sequence requirements of the HIV-1 protease flap region determined by saturation mutagenesis and kinetic analysis of flap mutants. The HIV-1 Protease as a Therapeutic Target …

Veröffentlicht in:Biochemistry · 1991Autoren:Mariusz Jaskolski · Alfredo G Tomasselli · Tomi K Sawyer · Douglas G Staples · Robe…Über:Enzyme inhibitor · Active site · Crystal structure · Virus

Large-Scale Structure-Based Prediction and Identification

In silico prediction of mutant HIV-1 proteases cleaving a target sequence Jensen, J.H.; Willemoës, M.; Winther, J.R.; De Vico, L. Predicting the tolerated sequences for proteins and protein interfaces using RosettaBackrub flexible backbone design

Commercial proteases: Present and future – Li – 2013

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This review presents a brief overview of the general categories of commercially used proteases, and critically surveys the successful strategies currently being used to improve the properties of proteases for various commercial purposes.

Hypothesis on Serenoa repens (Bartram) small – PeerJ

In silico prediction of mutant HIV-1 proteases cleaving a target sequence. PLoS ONE 9 : e95833 Jez JM , Bennett MJ , Schlegel BP , Lewis M , Penning TM . 1997 .